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Life Science Research Professional

 
The Department of Neurosurgery at Stanford is seeking a diligent research assistant to establish a surgical specimen database independently and work on several projects supervised by our lab manager and postdoctoral fellows.
 
1.   Database establishment and management: Fresh Tissue banking inventory: Establishing and maintaining an inventory system to track fresh tissue samples from craniotomies and autopsies in the Petritsch lab. A software license for Freezerworks Ascent version was purchased and now needs to be set up to be used by Petritsch lab members and linked to REDCap database containing patient information (derived from EPIC) and molecular and pathology data.
a.    In charge of supervising the installment of the FreezerWorks tissue banking inventory software on the remote server at Stanford
b.    Working with Stanford IT, FreezerWorks, Neuroscience Tissue coordinator to install user licenses and harmonize access
c.    Work with other labs tissue bank staff to unify record keeping
d.    Costumize Freezerworks for the Petritsch lab to track frozen tissue pieces, single cell suspensions, xenografted cells
e.   link RedCap patient database with Freezerworks
f.     Data entry and maintenance
g.    Help other users (i.e. labs from NS and Neurology to get started with FW software)
2.    Fresh tissue collection from OR for research (model development and cell-based assays and -omics analyses.
a.    Discuss with postdoc which tissue to collect
b.    Familiarize with all protocols related to CNS tumor and tissue collection and processing
c.     Actively participate in collecting tissue from the NS-OR from craniotomies in children and coordinate with to pick up fresh tissue from OR
d.    Coordinate with other interested parties to distribute tissue
e.    Communicate with OR staff while ensuring patient safety
f.     Pick up tissue from OR and rapidly process in TC room (dissection, removal of debris and RBC etc..)
g.    Plate cells and freeze samples in timely manner and using the FW tracking and labeling system
3.    Expanding, maintaining animal colony
a.    Checking the mice and perform weaning
b.    Cutting tails, toe tattooing, submitting samples for genotyping, interpreting results and updating mouse database, communication with lab members to decide about experiments, which animals to keep
4.    Strategize experiments with postdoc and help with their experiments in whatever way they need, including processing of animals and tissue for sectioning, immunofluorescence, confocal imaging, data analyses, dosing of mice, cell culturing for animal experiments, testing drug responses in vitro and in vivo, assist with small animal surgeries, RNA/DNA preparation, magnetic and flow cytometry sorting for cell populations, transcriptional profiling, RNA-seq, hESC differentiation, Cripsr/CAS-mediated genome editing and introducing glioma-inducing alterations.